Ovulation timing is probably the most important aspect to a successful breeding regardless of the type of semen you use or the method of insemination used.

Several things to take into account when thinking of using frozen semen

Where is the frozen semen stored that you are planning to use?  Is it already stored with your reproduction specialist or does it need to be shipped in?  If it is not stored with your specialist, will you need to pay release and shipping fees to get it to your Dr.

You need to do progesterone timing as it is imperative that the semen be placed in the bitch at the correct time as it will only live for 12 hours once it is thawed.  Generally, you are looking to inseminate day 5 or day 6 post LH surge, approximately 60 hours post ovulation and at progesterone values of 35 – 40 ng/ml using miniVidas results.

How much frozen semen do you have access to?  Do you only have one breeding unit available or can you get two.

What method of insemination do you want to use?  With frozen semen it is recommended that it be implanted surgically due to its shortened life of 12 hours.  If you have access to two breeding units, it is recommended that you do a TCI followed up 12 hours later with a surgical.  This gives you 24 hours of coverage in which to get the eggs fertilized.  It is not generally recommended that frozen semen be inserted via a Vaginal AI.  You can have success with this method but specialist have found that you get a much lower rate of conception and smaller sized litters.